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Addgene

Nanog-2A-mCherry
(Plasmid #48680)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48680 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1TOPO
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nanog
  • Alt name
    ENK
  • Alt name
    ecat4
  • Alt name
    2410002E02Rik
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3000
  • GenBank ID
    NC_000072.6
  • Entrez Gene
    Nanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)
  • Tag / Fusion Protein
    • Cherry (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nanog-2A-mCherry was a gift from Rudolf Jaenisch (Addgene plasmid # 48680 ; http://n2t.net/addgene:48680 ; RRID:Addgene_48680)

  • For your References section:

    One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering. Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R. Cell. 2013 Aug 27. pii: S0092-8674(13)01016-7. doi: 10.1016/j.cell.2013.08.022. 10.1016/j.cell.2013.08.022 PubMed 23992847
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