pDONR P2R-P3-SV40 polyA signal
(Plasmid #48627)

• Purpose: Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.
• Depositing Labs: Anna Planas, Tomas Santalucia
• Publication: Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.

Backbone

• Vector backbone: pDONRP2R-P3
• Backbone manufacturer: Life Technologies/Invitrogen
• Backbone size w/o insert (bp): 2640
• Total vector size (bp): 2773
• Vector type: Gateway entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SV40 early polyadenylation signal cassette
• Insert Size (bp): 133
• Promoter: none

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 forward (-20)
• 3′ sequencing primer: M13 reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Life Technologies

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry