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PurposeContributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONRP2R-P3
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Backbone manufacturerLife Technologies/Invitrogen
- Backbone size w/o insert (bp) 2640
- Total vector size (bp) 2773
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Vector typeGateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSV40 early polyadenylation signal cassette
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Insert Size (bp)133
- Promoter none
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 forward (-20)
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byLife Technologies
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR P2R-P3-SV40 polyA signal was a gift from Anna Planas & Tomas Santalucia (Addgene plasmid # 48627 ; http://n2t.net/addgene:48627 ; RRID:Addgene_48627) -
For your References section:
A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector. Buj R, Iglesias N, Planas AM, Santalucia T. BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18. 10.1186/1471-2199-14-18 PubMed 23957834
Included in kit:
Commonly requested with:
Map uploaded by the depositor.
