pDONR P4-P1R-EGFP
(Plasmid #48348)

• Purpose: Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
• Depositing Labs: Anna Planas, Tomas Santalucia
• Publication: Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.

Backbone

• Vector backbone: pDONRP4-P1R
• Backbone manufacturer: Life Technologies/Invitrogen
• Backbone size w/o insert (bp): 2655
• Total vector size (bp): 3363
• Vector type: Gateway entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Species: Synthetic; Aequorea victoria
• Insert Size (bp): 708
• Mutation: Contains a Kozak sequence. Does not contain a stop codon. Aminoacid 40 is valine in our sequence, while it is phenylalanine in other EGFP sequences. This does not seem to affect fluorescence.
• Promoter: none

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 forward (-20)
• 3′ sequencing primer: M13 reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Clontech

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry