pAC149-pCR8-dCas9VP160
(Plasmid #48221)

• Purpose: dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
• Depositing Lab: Rudolf Jaenisch
• Publication: Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.

Backbone

• Vector backbone: pCR8/GW/TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2799
• Total vector size (bp): 7442
• Vector type: CRISPR ; Gateway Donor

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dCas9(D10A;H840A) fusion with VP160 activation domain
• Alt name: dCas9VP160
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 4643
• Mutation: D10A;H840A nuclease-deficient
• Tags / Fusion Proteins:
  • HA Tag (N terminal on insert)
  • VP160 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • Genbank File
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information including protocols and
updates, please go to http://www.crispr-on.org