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pAC149-pCR8-dCas9VP160
(Plasmid #48221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR8/GW/TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2799
  • Total vector size (bp) 7442
  • Vector type
    CRISPR ; Gateway Donor

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9(D10A;H840A) fusion with VP160 activation domain
  • Alt name
    dCas9VP160
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    4643
  • Mutation
    D10A;H840A nuclease-deficient
  • Tags / Fusion Proteins
    • HA Tag (N terminal on insert)
    • VP160 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information including protocols and
updates, please go to http://www.crispr-on.org

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC149-pCR8-dCas9VP160 was a gift from Rudolf Jaenisch (Addgene plasmid # 48221 ; http://n2t.net/addgene:48221 ; RRID:Addgene_48221)

  • For your References section:

    Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. 10.1038/cr.2013.122 PubMed 23979020
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