Skip to main content
Addgene

pSP6-RNAP
(Plasmid #48143)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48143 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMGBm19
  • Backbone manufacturer
    Gamer et al., 2009
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    For Bacillus megaterium: Chloramphenicol and 37 °C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rna polymerase SP6
  • Mutation
    V314A (numbering based on NCBI Sequence ID: NP_853568.1)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the V313A mutation in RNAP was present in the original sample and functions as described in the publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP6-RNAP was a gift from Dieter Jahn (Addgene plasmid # 48143 ; http://n2t.net/addgene:48143 ; RRID:Addgene_48143)

  • For your References section:

    Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2. 10.1007/s00253-010-2732-y PubMed 20596705
Related items:
Commonly requested with: