pSPPac-hp
(Plasmid
#48123)
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PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48123 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMM1520
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Backbone manufacturerMalten et al., 2005
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Growth instructionsFor Bacillus megaterium: Tetracycline and 37 °C
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesignal peptide of Pac
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSPPac-hp was a gift from Dieter Jahn (Addgene plasmid # 48123 ; http://n2t.net/addgene:48123 ; RRID:Addgene_48123) -
For your References section:
High-yield intra- and extracellular protein production using Bacillus megaterium. Stammen S, Muller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara E, Jahn D. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30. 10.1128/AEM.00431-10 PubMed 20435764
Map uploaded by the depositor.
