pAGM4723
(Plasmid #48015)

• Purpose: (Empty Backbone) Please see depositor comments section.
• Depositing Lab: Sylvestre Marillonnet
• Publication: Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765.

Backbone

• Vector backbone: pAGM4351
• Backbone manufacturer: self-made
• Backbone size (bp): 4824
• Vector type: Unspecified
• Selectable markers: Canthaxanthin operon

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE NOTE- It has recently come to light that plasmid pAGM4723 lacks a complete overdrive sequence at the right border. This can cause incomplete TDNA insertion events and can give a higher incidence of vector backbone transfer events due to transfer initiating at the left border.

Level 2 cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells.

Please note that bacterial colonies for this plasmid often appear red in color because the plasmid contains a red color selectable marker (CRed--an artificial bacterial operon responsible for canthaxanthin biosynthesis).