pMB60
(Plasmid #47941)

• Purpose: Can be used to generate synthetic guide in vitro from T7 promotor
• Depositing Lab: Mike Boxem
• Publication: Waaijers et al Genetics. 2013 Aug 26.

Backbone

• Vector backbone: pMK
• Backbone manufacturer: Geneart
• Total vector size (bp): 2286
• Vector type: Worm Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: synthetic guide RNA
• Alt name: sgRNA
• Species: Synthetic
• Insert Size (bp): 80

Resource Information

• Supplemental Documents:
  • Snapgene file
  • Genbank file
  • Features
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Boxem Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/boxem/