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PurposeFLAG tagged NmCas9 with no NLS.
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47872 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSimpleII
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 8501
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNmCas9
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Alt nameCSN1
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SpeciesN meningitidis
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Insert Size (bp)3243
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSimpleII-NmCas9-FLAG was a gift from Erik Sontheimer & James Thomson (Addgene plasmid # 47872 ; http://n2t.net/addgene:47872 ; RRID:Addgene_47872) -
For your References section:
Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA. Proc Natl Acad Sci U S A. 2013 Aug 12. 10.1073/pnas.1313587110 PubMed 23940360
Map uploaded by the depositor.
