pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
(Plasmid #47868)

• Purpose: This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
• Depositing Labs: Erik Sontheimer, James Thomson
• Publication: Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Backbone

• Vector backbone: pSimpleII
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 8501
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: NmCas9
• Alt name: CSN1
• Species: N meningitidis
• Insert Size (bp): 3243
• Promoter: EF1a
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)
  • HA (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: U6pr-tracrRNA
• Species: N meningitidis
• Insert Size (bp): 364
• Promoter: U6

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • GeneBank File
  • cloning instruction
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry