pBK-CMV-hNHERF2
(Plasmid #47801)

• Purpose: Expresses human NHERF2 in mammalian cells
• Depositing Lab: Randy Hall
• Publication: Lau et al Biochemistry. 2001 Jul 24;40(29):8572-80.

Backbone

• Vector backbone: modified pBK-CMV
• Backbone manufacturer: Stratagene
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NHERF2
• Alt name: SLC9A3R2
• Alt name: SIP1
• Alt name: solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2
• Species: H. sapiens (human)
• Entrez Gene: NHERF2 (a.k.a. E3KARP, NHE3RF2, NHERF-2, OCTS2, SIP-1, SIP1, SLC9A3R2, TKA-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13_pUC_rev_primer
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry