pSL690
(Plasmid #47753)

• Purpose: Expresses dCas9-VP64 fusion
• Depositing Lab: Keith Joung
• Publication: Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598.

Backbone

• Vector backbone: unknown
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dCas9-VP64
• Alt name: inactive Cas9 nuclease
• Alt name: VP64 activation domain
• Species: Synthetic; Streptococcus pyogenes
• Mutation: D10A and H840A mutations in Cas9
• Promoter: CMV
• Tags / Fusion Proteins:
  • NLS (C terminal on insert)
  • 3x FLAG (C terminal on insert)
  • VP64 (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: dCas9 amplified from Jennifer Doudna lab--plasmid pMJ841 (Addgene plasmid # 39318)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid Features:
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
VP64 = 4633-4794