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Addgene

EGFPN3-hMyoX
(Plasmid #47609)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47609 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EGFPN3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Modifications to backbone
    The KpnI-BglII fragment containing hMyoX was inserted into the KpnI-BamHI sites of the pEGFP-N3 vector. This cloning strategy destroyed the BglII and BamHI sites.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MyoX
  • Alt name
    MYO10
  • Alt name
    myosin X
  • Species
    H. sapiens (human)
  • Mutation
    Q680R
  • GenBank ID
    AF234532.1
  • Entrez Gene
    MYO10 (a.k.a. MyoX)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFPN3-hMyoX was a gift from Emanuel Strehler (Addgene plasmid # 47609 ; http://n2t.net/addgene:47609 ; RRID:Addgene_47609)
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