pcDNA4TO-hCALML3
(Plasmid
#47601)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47601 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA4/TO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCALM3
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Alt namecalmodulin-like 3
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Alt nameCLP
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SpeciesH. sapiens (human)
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Entrez GeneCALML3 (a.k.a. CLP)
- Promoter tet-inducible CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
tetracycline-inducible expression of CLP
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4TO-hCALML3 was a gift from Emanuel Strehler (Addgene plasmid # 47601 ; http://n2t.net/addgene:47601 ; RRID:Addgene_47601) -
For your References section:
Calmodulin-like protein increases filopodia-dependent cell motility via up-regulation of myosin-10. Bennett RD, Mauer AS, Strehler EE. J Biol Chem. 2007 Feb 2;282(5):3205-12. Epub 2006 Nov 26. 10.1074/jbc.M607174200 PubMed 17130134