pCDFDuet-1 MKK4(EE)-MKK7a1(EE)
(Plasmid
#47580)
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PurposeBacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47580 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDFDuet-1
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3781
- Total vector size (bp) 5851
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BL21
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameMKK4-EE
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Alt nameMAP2K4
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Alt nameMEK4
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SpeciesH. sapiens (human)
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Insert Size (bp)1092
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MutationLacks the first 36 residues of the RefSeq protein. Ser257 to Glu, Thr261 to Glu
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GenBank IDNM_003010
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Entrez GeneMAP2K4 (a.k.a. JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1)
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer ACYCDuetUP1
- 3′ sequencing primer DuetDOWN1 (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameMKK7a1-EE
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Alt nameMap2k7
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Alt nameMek7
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SpeciesM. musculus (mouse)
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Insert Size (bp)1041
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MutationResidues 73-420 of RefSeq sequence. Ser271 to Glu, Thr275 to Glu.
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GenBank IDNM_001042557
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer DuetUP2 Primer
- 3′ sequencing primer T7 Terminator Primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene plasmids #47577 and #47578.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDFDuet-1 MKK4(EE)-MKK7a1(EE) was a gift from Kevin Janes (Addgene plasmid # 47580 ; http://n2t.net/addgene:47580 ; RRID:Addgene_47580) -
For your References section:
A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts. Bose AK, Janes KA. Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 10.1074/mcp.O112.024059. Epub 2012 Dec 11. 10.1074/mcp.O112.024059 PubMed 23233447