Flag Intersectin Short (human)
(Plasmid #47392)

• Depositing Lab: Peter McPherson
• Publication: Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4754
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Intersectin short
• Alt name: intersectin-s
• Alt name: ITSN1
• Species: H. sapiens (human)
• Entrez Gene: ITSN1 (a.k.a. ITSN, SH3D1A, SH3P17)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry