pWH-DI
(Plasmid
#47385)
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PurposeExpresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47385 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepWH1520
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Backbone manufacturerMo Bi Tec
- Backbone size w/o insert (bp) 7705
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namedxs
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Alt name1-deoxy-D-xylulose-5-phosphate synthase
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Alt nameDXP-synthase
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SpeciesB. subtilis
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Insert Size (bp)1899
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GenBank ID938609
- Promoter PxylA
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer NA (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameidi
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Alt nameisopentenyl-diphosphate delta-isomerase
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SpeciesB. subtilis
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Insert Size (bp)1050
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GenBank ID11239955
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWH-DI was a gift from Heng-Phon Too (Addgene plasmid # 47385 ; http://n2t.net/addgene:47385 ; RRID:Addgene_47385) -
For your References section:
Optimization of amorphadiene synthesis in Bacillus subtilis via transcriptional, translational, and media modulation. Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP. Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900. 10.1002/bit.24900 PubMed 23483530
Map uploaded by the depositor.
