pWH-DI
(Plasmid #47385)

• Purpose: Expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria
• Depositing Lab: Heng-Phon Too
• Publication: Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.

Backbone

• Vector backbone: pWH1520
• Backbone manufacturer: Mo Bi Tec
• Backbone size w/o insert (bp): 7705
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: dxs
• Alt name: 1-deoxy-D-xylulose-5-phosphate synthase
• Alt name: DXP-synthase
• Species: B. subtilis
• Insert Size (bp): 1899
• GenBank ID: 938609
• Promoter: PxylA

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: NA

Gene/Insert 2

• Gene/Insert name: idi
• Alt name: isopentenyl-diphosphate delta-isomerase
• Species: B. subtilis
• Insert Size (bp): 1050
• GenBank ID: 11239955

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry