Skip to main content
Addgene

pBS-HA::UPRT
(Plasmid #47110)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47110 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript SK-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 3792

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    uracil phosphoribosyltransferase
  • Alt name
    UPRT
  • Species
    Toxoplasma gondii
  • Insert Size (bp)
    792
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer M13 Reverse
  • 3′ sequencing primer T7 Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-HA::UPRT was a gift from Mike Cleary & Chris Doe (Addgene plasmid # 47110 ; http://n2t.net/addgene:47110 ; RRID:Addgene_47110)

  • For your References section:

    TU-tagging: cell type-specific RNA isolation from intact complex tissues. Miller MR, Robinson KJ, Cleary MD, Doe CQ. Nat Methods. 2009 Jun;6(6):439-41. doi: 10.1038/nmeth.1329. Epub 2009 May 10. 10.1038/nmeth.1329 PubMed 19430475
Related items:
Commonly requested with: