pBXC3H
(Plasmid
#47068)
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Purpose(Empty Backbone) FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47068 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD24
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Backbone manufacturerLM Guzman
- Backbone size (bp) 5780
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Modifications to backbonemodified for compatibility with FX cloning and added tags/fusion proteins
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Vector typeBacterial Expression
- Promoter araBAD
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Tags
/ Fusion Proteins
- 3C protease site (PreScission site) (C terminal on backbone)
- decaHis (10x His) (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberUnknown
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBAD-F
- 3′ sequencing primer pBAD-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBXC3H was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 47068 ; http://n2t.net/addgene:47068 ; RRID:Addgene_47068) -
For your References section:
A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291
Included in kit:
Commonly requested with:
