p7XC3GH
(Plasmid #47066)

• Purpose: (Empty Backbone) FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags
• Depositing Labs: Raimund Dutzler, Eric Geertsma
• Publication: Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.

Backbone

• Vector backbone: pET26b
• Backbone manufacturer: Novagen
• Backbone size (bp): 7721
• Modifications to backbone: modified for compatibility with FX cloning and added tags/fusion proteins
• Vector type: Bacterial Expression
• Promoter: T7
• Tags / Fusion Proteins:
  • 3C protease site (PreScission site) (C terminal on backbone)
  • GFP (C terminal on backbone)
  • decaHIS (10x His) (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: Unknown

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry