pJBEI-6411
(Plasmid #47050)

• Purpose: BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli
• Depositing Lab: Taek Soon Lee
• Publication: Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.

Backbone

• Vector backbone: pBbB8k
• Backbone size w/o insert (bp): 2300
• Total vector size (bp): 6656
• Vector type: Bacterial Expression, Synthetic Biology ; BglBrick

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
• Species: Sphingomonas
• Insert Size (bp): 4358
• Promoter: Pbad

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI, BglII (not destroyed)
• 3′ cloning site: BamHI, XhoI (not destroyed)
• 5′ sequencing primer: F8
• 3′ sequencing primer: R1

Resource Information

• Supplemental Documents:
  • Plasmid map
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use a low concentration of Kanamycin (12.5 micrograms/mililiter) for perillyl alcohol production