pAAV-MCS2
(Plasmid #46954)

• Purpose: (Empty Backbone) Cloning plasmid for the generation of Knock-In in human cells using rAAV.
• Depositing Lab: Steve Jackson
• Publication: Britton et al J Cell Biol. 2013 Jul 29.

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Backbone

• Vector backbone: pAAV-MCS
• Backbone manufacturer: Agilent Technologies
• Backbone size (bp): 4650
• Modifications to backbone: A 1,345-bp fragment of Ku80 cDNA was digested with NotI–Bsp12OIand cloned as a linker flanked by restrictions sites into NotI-digested pAAV-MCS.
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • Author annotated GenBank sequence
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene verified sequence found an 11bp deletion in one ITR and D158G in the Ku80 sequence. The depositor confirmed that this does not impact function.