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pCMV4Neo-murine IL-17RASEFIR-HA
(Plasmid #46863)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46863 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV4-Neo
  • Backbone size w/o insert (bp) 5750
  • Total vector size (bp) 8100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IL-17RA-delta-SEFIR
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • Mutation
    Deletion of SEFIR domain (AA 394-485)
  • Entrez Gene
    Il17ra (a.k.a. AW538159, Cdw217, Il17r, VDw217)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3x HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba (upstream of 3xHA site) (not destroyed)
  • 5′ sequencing primer GAACCGTCAGAATTG
  • 3′ sequencing primer CTTCCAAGGCCAGGAGAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV4Neo-murine IL-17RASEFIR-HA was a gift from Sarah Gaffen (Addgene plasmid # 46863 ; http://n2t.net/addgene:46863 ; RRID:Addgene_46863)

  • For your References section:

    Distinct functional motifs within the IL-17 receptor regulate signal transduction and target gene expression. Maitra A, Shen F, Hanel W, Mossman K, Tocker J, Swart D, Gaffen SL. Proc Natl Acad Sci U S A. 2007 May 1;104(18):7506-11. Epub 2007 Apr 24. 10.1073/pnas.0611589104 PubMed 17456598
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