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Purpose(Empty Backbone) Vector modified from pBluescript SK(+) by adding more cloning sites
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46848 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript SK(+)
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Backbone manufacturerStratagene
- Backbone size (bp) 2960
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Modifications to backboneAdd FseI, PacI, BglII and AscI restriction site between KpnI and XhoI in pBluescript SK(+)vector
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Vector typeCloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pX1 was a gift from Monica Hollstein (Addgene plasmid # 46848 ; http://n2t.net/addgene:46848 ; RRID:Addgene_46848) -
For your References section:
Rapid derivation of genetically related mutants from embryonic cells harboring a recombinase-specific Trp53 platform. Wei QX, Odell AF, van der Hoeven F, Hollstein M. Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15. 10.4161/cc.10.8.15303 PubMed 21445009
Map uploaded by the depositor.
