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Addgene

pLIGFP_W220F
(Plasmid #46772)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46772 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET22b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5493
  • Total vector size (bp) 6093
  • Modifications to backbone
    Position 220 in LacI is mutated from tryptophan to phenylalanine

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LacI_W220F
  • Alt name
    LacIWF
  • Alt name
    LacI
  • Insert Size (bp)
    1083
  • Mutation
    Tryptophan 220 replaced by Phenylalanine
  • Entrez Gene
    lacI (a.k.a. b0345, ECK0342)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer cgccattcgatggtgtcc
  • 3′ sequencing primer aggctctcaagggcatcgg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use BL21 for expression. Growth temperature depends on the protein being over-expressed and is not affected by the modified repressor LacIWF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIGFP_W220F was a gift from Florian Hollfelder (Addgene plasmid # 46772 ; http://n2t.net/addgene:46772 ; RRID:Addgene_46772)

  • For your References section:

    A single mutation in the core domain of the lac repressor reduces leakiness. Gatti-Lafranconi P, Dijkman WP, Devenish SR, Hollfelder F. Microb Cell Fact. 2013 Jul 8;12:67. doi: 10.1186/1475-2859-12-67. 10.1186/1475-2859-12-67 PubMed 23834731
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