pWJ66
(Plasmid #46570)

• Purpose: Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
• Depositing Lab: Luciano Marraffini
• Publication: Bikard et al Nucleic Acids Res. 2013 Jun 12.

Backbone

• Vector backbone: pACYC184
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: tracrRNA
• Species: S. pyogenes

Gene/Insert 2

• Gene/Insert name: dcas9-w
• Species: S. pyogenes
• Mutation: D10A, H840A
• GenBank ID: Spy_1046
• Tag / Fusion Protein:
  • rpoZ (C terminal on insert)

Gene/Insert 3

• Gene/Insert name: CRISPR array

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was constructed by modifying the pCas9 plasmid constructed in the lab of Feng Zhang at MIT, and published in Nat Biotechnol. 2013 Mar;31(3):233-9. doi: 10.1038/nbt.2508. RNA-guided editing of bacterial genomes using CRISPR-Cas systems
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry