pENTR FL CPAP RNAi resistant
(Plasmid
#46390)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46390 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepENTR 1A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2717
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Vector typeEntry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCPAP RNAi resistant mutant
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Alt namecentrosome protein 4.1 associated protein
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Alt nameCENPJ
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Alt nameCPAP
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SpeciesH. sapiens (human)
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Mutationsilent mutation that renders it resistant to siRNA :nucleotides 2862–2880, new sequence: 5′-AGAGTTAGCTAGGATCGAAGA-3′
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Entrez GeneCENPJ (a.k.a. BM032, CENP-J, CPAP, LAP, LIP1, MCPH6, SASS4, SCKL4, Sas-4)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pENTR-F
- 3′ sequencing primer pENTR-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR FL CPAP RNAi resistant was a gift from Pierre Gonczy (Addgene plasmid # 46390 ; http://n2t.net/addgene:46390 ; RRID:Addgene_46390) -
For your References section:
Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL. Kitagawa D, Kohlmaier G, Keller D, Strnad P, Balestra FR, Fluckiger I, Gonczy P. J Cell Sci. 2011 Nov 15;124(Pt 22):3884-93. doi: 10.1242/jcs.089888. Epub 2011 Nov 18. 10.1242/jcs.089888 PubMed 22100914