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Addgene

pHIS2-KL EAT
(Plasmid #46042)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46042 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHIS-parallel.2
  • Backbone size w/o insert (bp) 5550
  • Total vector size (bp) 6360
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Atg1 EAT domain
  • Alt name
    Atg1 CTD
  • Species
    K. lactis
  • Insert Size (bp)
    810
  • GenBank ID
    CAH01818.1
  • Tag / Fusion Protein
    • HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-ter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIS2-KL EAT was a gift from James Hurley (Addgene plasmid # 46042 ; http://n2t.net/addgene:46042 ; RRID:Addgene_46042)

  • For your References section:

    Architecture of the Atg17 complex as a scaffold for autophagosome biogenesis. Ragusa MJ, Stanley RE, Hurley JH. Cell. 2012 Dec 21;151(7):1501-12. doi: 10.1016/j.cell.2012.11.028. Epub 2012 Dec 6. 10.1016/j.cell.2012.11.028 PubMed 23219485
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