tetO-MEF2C
(Plasmid #46031)

• Depositing Lab: John Gearhart
• Publication: Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004.

Backbone

• Vector backbone: tetO-Gateway
• Total vector size (bp): 10000
• Modifications to backbone: MEF2C cloned into Gateway site of tetO-Gateway
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MEF2C
• Species: H. sapiens (human)
• Insert Size (bp): 1450
• GenBank ID:
• Entrez Gene: MEF2C (a.k.a. C5DELq14.3, DEL5q14.3, NEDHSIL)
• Promoter: tetO
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry