tetO-GATA4
(Plasmid #46030)

• Depositing Lab: John Gearhart
• Publication: Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004.

Backbone

• Vector backbone: tetO-Gateway
• Total vector size (bp): 10000
• Modifications to backbone: GATA4 cloned into Gateway site of tetO-Gateway
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GATA4
• Species: H. sapiens (human)
• Insert Size (bp): 1454
• Mutation: R43Q, G50S and N197S
• GenBank ID:
• Entrez Gene: GATA4 (a.k.a. ASD2, TACHD, TOF, VSD1)
• Promoter: tetO
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing identified R43Q, G50S and N197S mutations in the GATA4 insert in this plasmid when compared to GenBank reference sequence NP_002043.2. These difference were present in the original GATA4 sample and the plasmid functions as described in the associated publication.