TroponinT-GCaMP5-Zeo
(Plasmid #46027)

• Depositing Lab: John Gearhart
• Publication: Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004.

Backbone

• Vector backbone: TroponinT-Gateway
• Total vector size (bp): 10565
• Modifications to backbone: GCaMP5-2A-Zeo cloned into TroponinT-Gateway using LR Recombinase.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GCaMP5-2A-Zeo
• Species: Synthetic
• Insert Size (bp): 1800
• Promoter: TNNT2

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified nucleotide mismatches at bp# 3408, 4035, 4136 & 4641 when compared to the full plasmid sequence. According to the depositing laboratory, these differences are not a concern for the function of the plasmid.