pCM351
(Plasmid #46013)

• Purpose: (Empty Backbone)
• Depositing Lab: Mary Lidstrom
• Publication: Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

Backbone

• Vector backbone: pAYC61
• Backbone manufacturer: Lidstrom Lab (PMID 8021187)
• Backbone size (bp): 6000
• Modifications to backbone: loxP-flanked gentamicin-resistance cassette (encoded by aaaC1) was amplified from pLoxGen4 (Palmeros, 2000), using CM-ufkMCS and CM-dfkMCS (see Addgene plasmid 46012 for sequences), and cloned into pCR2.1 (Invitrogen) to produce pCM350. The 1-kb AatII/SacI fragment from pCM350 was cloned between the AatII and SacI sites of pCM184 (Addgene plasmid 46012) to generate pCM351.
• Vector type: Cre/Lox
• Promoter: na

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This allelic exchange vector is used with Cre expression vectors (Addgene plasmids 45863 or 45864) to generate unmarked mutants in a broad bacterial host range.