pGSH0
(Plasmid
#45500)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45500 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGSH0
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Backbone manufacturerLau lab
- Backbone size (bp) 5229
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Vector typeMammalian Expression, RNAi
- Promoter CMV
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Tag
/ Fusion Protein
- GFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer ttgagggagacaccctcg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGSH0 was a gift from Nelson Lau (Addgene plasmid # 45500 ; http://n2t.net/addgene:45500 ; RRID:Addgene_45500) -
For your References section:
Organic small hairpin RNAs (OshR): A do-it-yourself platform for transgene-based gene silencing. Zeng M, Kuzirian MS, Harper L, Paradis S, Nakayama T, Lau NC. Methods. 2013 May 23. pii: S1046-2023(13)00148-5. doi: 10.1016/j.ymeth.2013.05.007. 10.1016/j.ymeth.2013.05.007 PubMed 23707624
Map uploaded by the depositor.
