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Addgene

pGEMABC
(Plasmid #45398)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45398 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEMEX1
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3993
  • Total vector size (bp) 12737
  • Modifications to backbone
    Digesting pGEMEX1 (Promega) with Xbal and recircularizing the larger fragment. By this treatment, the T7 gene 10 coding for major capsid protein was deleted.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    rpoA
  • Alt name
    alpha subunit RNA polymerase
  • Species
    Escherichia coli
  • Insert Size (bp)
    987
  • Entrez Gene
    rpoA (a.k.a. Y75_p3881)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer None
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    rpoB
  • Alt name
    beta subunit RNA polymerase
  • Species
    Escherichia coli
  • Insert Size (bp)
    4026
  • Entrez Gene
    rpoB (a.k.a. Y75_p3210)
  • Promoter None

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer None
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    rpoC
  • Alt name
    betaprime subunit RNA polymerase
  • Species
    Escherichia coli
  • Insert Size (bp)
    4221
  • Entrez Gene
    rpoC (a.k.a. Y75_p3209)
  • Promoter None

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMABC was a gift from Katsu Murakami (Addgene plasmid # 45398 ; http://n2t.net/addgene:45398 ; RRID:Addgene_45398)

  • For your References section:

    X-ray Crystal Structure of Escherichia coli RNA Polymerase sigma70 Holoenzyme. Murakami KS. J Biol Chem. 2013 Mar 29;288(13):9126-34. doi: 10.1074/jbc.M112.430900. Epub 2013 Feb 6. 10.1074/jbc.M112.430900 PubMed 23389035
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