pBabe_puro_DEST_Flag_PHC3
(Plasmid
#45262)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45262 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe-puro-flag-DEST
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePHC3
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SpeciesH. sapiens (human)
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Entrez GenePHC3 (a.k.a. EDR3, HPH3)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pBabe5'
- 3′ sequencing primer pBabe3' (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byTHE CCSB HUMAN ORFEOME COLLECTION
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe_puro_DEST_Flag_PHC3 was a gift from William Hahn (Addgene plasmid # 45262 ; http://n2t.net/addgene:45262 ; RRID:Addgene_45262) -
For your References section:
Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer drivers. Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P, Mesirov JP, Beroukhim R, Hahn WC. Cancer Discov. 2013 Jun 13. [Epub ahead of print] 10.1158/2159-8290.CD-12-0592 PubMed 23764425