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Addgene

LacI INCENP
(Plasmid #45236)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45236 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LacI, INCENP-Δcen, mCherry
  • Alt name
    pXW029
  • Mutation
    CEN box deleted from INCENP (aa1-46)
  • Entrez Gene
    lacI (a.k.a. b0345, ECK0342)
  • Promoter t7
  • Tags / Fusion Proteins
    • mCherry
    • LacI (lac repressor protein)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer t7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LacI INCENP was a gift from Michael Lampson (Addgene plasmid # 45236 ; http://n2t.net/addgene:45236 ; RRID:Addgene_45236)

  • For your References section:

    Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. Wang E, Ballister ER, Lampson MA. J Cell Biol. 2011 Aug 22;194(4):539-49. doi: 10.1083/jcb.201103044. Epub 2011 Aug 15. 10.1083/jcb.201103044 PubMed 21844210
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