pDZ416 (24PP7SL loxP-Kan-loxP)
(Plasmid #45163)

• Depositing Labs: Robert Singer, Daniel Zenklusen
• Publication: Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 2012 Dec 23.

Backbone

• Vector backbone: pKAN
• Vector type: Cre/Lox
• Selectable markers: Geneticin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 24xPP7 Stem Loop Cassette
• Species: Synthetic
• Insert Size (bp): 1453

Resource Information

• Supplemental Documents:
  • Map
  • annotated sequence file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP. See associated publication for detailed methods and use of this plasmid.