pBS-Histone H1
(Plasmid
#45083)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45083 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II SK(+)
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Backbone manufacturerAgilent Technologies
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 3800
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Vector typeRNAi ; in vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHistone H1
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Alt nameH1
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Alt nameHistoneH1
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Alt namePL04013A1A11
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SpeciesSchmidtea mediterranea
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Insert Size (bp)781
- Promoter T7 sense, T3 antisense
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer M13 –20
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-Histone H1 was a gift from Phillip Newmark (Addgene plasmid # 45083 ; http://n2t.net/addgene:45083 ; RRID:Addgene_45083) -
For your References section:
PIWI homologs mediate histone H4 mRNA localization to planarian chromatoid bodies. Rouhana L, Weiss JA, King RS, Newmark PA. Development. 2014 Jul;141(13):2592-601. doi: 10.1242/dev.101618. Epub 2014 Jun 5. 10.1242/dev.101618 PubMed 24903754