pLAC
(Plasmid #45072)

• Purpose: lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide
• Depositing Lab: Gordon Laurie
• Publication: Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700. Epub 2006 Aug 21.

Backbone

• Vector backbone: pTYB1
• Backbone manufacturer: New England Biolabs, INC
• Backbone size w/o insert (bp): 7477
• Total vector size (bp): 7794
• Vector type: Bacterial Expression ; Protein Expression Vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ER2566
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lacritin
• Alt name: LACRT
• Alt name: pLAC
• Species: H. sapiens (human)
• Insert Size (bp): 363
• GenBank ID: NM_033277.1
• Entrez Gene: LACRT
• Promoter: T7
• Tag / Fusion Protein:
  • Intein (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a few differences in the vector backbone when compared to the full plasmid sequence. The depositing laboratory indicates that these differences are not a concern for the function of the plasmid.