C25-pLAC
(Plasmid
#45283)
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Purposelacritin-intein fusion plasmid coding for truncated lacritin without signal peptide
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTYB1
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Backbone manufacturerNew England Biolabs, INC
- Backbone size w/o insert (bp) 7477
- Total vector size (bp) 7718
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Vector typeBacterial Expression ; Protein Expression Vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ER2566
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepLAC-C25
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Alt nameC25
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Alt nameLacritin
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SpeciesH. sapiens (human)
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Insert Size (bp)288
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Mutationcontains aa20-113 of lacritin
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GenBank IDNM_033277.1
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Entrez GeneLACRT
- Promoter T7
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Tag
/ Fusion Protein
- Intein (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The last 25 amino acids of lacritin were deleted in this construct (aa 114-138).
Please note that Addgene's sequencing results identified a few differences in the vector backbone when compared to the full plasmid sequence. The depositing laboratory indicates that these differences are not a concern for the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C25-pLAC was a gift from Gordon Laurie (Addgene plasmid # 45283 ; http://n2t.net/addgene:45283 ; RRID:Addgene_45283) -
For your References section:
Restricted epithelial proliferation by lacritin via PKCalpha-dependent NFAT and mTOR pathways. Wang J, Wang N, Xie J, Walton SC, McKown RL, Raab RW, Ma P, Beck SL, Coffman GL, Hussaini IM, Laurie GW. J Cell Biol. 2006 Aug 28;174(5):689-700. Epub 2006 Aug 21. 10.1083/jcb.200605140 PubMed 16923831