GST-FUS 465-526
(Plasmid #44983)

• Depositing Lab: Robin Reed
• Publication: Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi: 10.1016/j.celrep.2012.08.025. Epub 2012 Sep 27.

Backbone

• Vector backbone: pGEX-6P-1
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 5200
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FUS (465-526aa)
• Alt name: TLS
• Alt name: fused in sarcoma
• Species: H. sapiens (human)
• Insert Size (bp): 200
• Mutation: contains amino acids 465-526
• GenBank ID:
• Entrez Gene: FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NOTE: The actual length of the insert between the BamHI and XhoI sites is about 450bp, but there is a stop codon at the end of the relevant FUS reading frame. A diagnostic digest with BamHI and XhoI will release a band over twice as large as the reported insert size.