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pIRESN2 H2B-GFP-uvr8at(2x)
(Plasmid #44970)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44970 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESneo2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5308
  • Total vector size (bp) 9133
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-GFP-UVR8(2x)
  • Alt name
    histone H2B
  • Alt name
    UV-B resistance 8
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Insert Size (bp)
    3825
  • Mutation
    tandem repeat of UVR8 (both have Methionine 1 deleted) linked by a flexible linker: GDSGDGKGGKGSGDGSGGDGSG
  • GenBank ID
    NM_021058 NM_125781.3
  • Entrez Gene
    UVR8 (a.k.a. AT5G63860, MGI19.7, MGI19_7, UVB-RESISTANCE 8)
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
  • Promoter pCMV IE
  • Tag / Fusion Protein
    • internal GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AflII (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRESN2 H2B-GFP-uvr8at(2x) was a gift from Thanos Halazonetis (Addgene plasmid # 44970 ; http://n2t.net/addgene:44970 ; RRID:Addgene_44970)

  • For your References section:

    Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells. Crefcoeur RP, Yin R, Ulm R, Halazonetis TD. Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800. 10.1038/ncomms2800 PubMed 23653191
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