pCas9_GFP Citations (151)
Originally described in: Enhanced efficiency of human pluripotent stem cell genome editing through replacing TALENs with CRISPRs.Ding Q, Regan SN, Xia Y, Oostrom LA, Cowan CA, Musunuru K Cell Stem Cell. 2013 Apr 4;12(4):393-4. doi: 10.1016/j.stem.2013.03.006. PubMed Journal
Articles Citing pCas9_GFP
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| Chromosomal translocations in human cells are generated by canonical nonhomologous end-joining. Ghezraoui H, Piganeau M, Renouf B, Renaud JB, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, Brunet E. Mol Cell. 2014 Sep 18;55(6):829-42. doi: 10.1016/j.molcel.2014.08.002. Epub 2014 Sep 4. PubMed |
| An efficient genotyping method for genome-modified animals and human cells generated with CRISPR/Cas9 system. Zhu X, Xu Y, Yu S, Lu L, Ding M, Cheng J, Song G, Gao X, Yao L, Fan D, Meng S, Zhang X, Hu S, Tian Y. Sci Rep. 2014 Sep 19;4:6420. doi: 10.1038/srep06420. PubMed |
| Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks. Renouf B, Piganeau M, Ghezraoui H, Jasin M, Brunet E. Methods Enzymol. 2014;546:251-71. doi: 10.1016/B978-0-12-801185-0.00012-X. PubMed |
| Genome editing for human gene therapy. Meissner TB, Mandal PK, Ferreira LM, Rossi DJ, Cowan CA. Methods Enzymol. 2014;546:273-95. doi: 10.1016/B978-0-12-801185-0.00013-1. PubMed |
| A distal locus element mediates IFN-gamma priming of lipopolysaccharide-stimulated TNF gene expression. Chow NA, Jasenosky LD, Goldfeld AE. Cell Rep. 2014 Dec 11;9(5):1718-28. doi: 10.1016/j.celrep.2014.11.011. Epub 2014 Dec 4. PubMed |
| A Sox2 distal enhancer cluster regulates embryonic stem cell differentiation potential. Zhou HY, Katsman Y, Dhaliwal NK, Davidson S, Macpherson NN, Sakthidevi M, Collura F, Mitchell JA. Genes Dev. 2014 Dec 15;28(24):2699-711. doi: 10.1101/gad.248526.114. PubMed |
| The Basic Helix-Loop-Helix Transcription Factor NEUROG3 Is Required for Development of the Human Endocrine Pancreas. McGrath PS, Watson CL, Ingram C, Helmrath MA, Wells JM. Diabetes. 2015 Jul;64(7):2497-505. doi: 10.2337/db14-1412. Epub 2015 Feb 3. PubMed |
| Myocardial Infarction-Associated SNP at 6p24 Interferes With MEF2 Binding and Associates With PHACTR1 Expression Levels in Human Coronary Arteries. Beaudoin M, Gupta RM, Won HH, Lo KS, Do R, Henderson CA, Lavoie-St-Amour C, Langlois S, Rivas D, Lehoux S, Kathiresan S, Tardif JC, Musunuru K, Lettre G. Arterioscler Thromb Vasc Biol. 2015 Jun;35(6):1472-1479. doi: 10.1161/ATVBAHA.115.305534. Epub 2015 Apr 2. PubMed |
| An alternative pluripotent state confers interspecies chimaeric competency. Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause MN, Nery JR, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim NY, Lajara J, Guillen P, Campistol JM, Esteban CR, Ross PJ, Saghatelian A, Ren B, Ecker JR, Izpisua Belmonte JC. Nature. 2015 May 21;521(7552):316-21. doi: 10.1038/nature14413. Epub 2015 May 6. PubMed |
| Generation of Isogenic Human iPS Cell Line Precisely Corrected by Genome Editing Using the CRISPR/Cas9 System. Grobarczyk B, Franco B, Hanon K, Malgrange B. Stem Cell Rev. 2015 Jun 10. PubMed |
Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9.
Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC.
Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001.
PubMed
Associated Plasmids |
| Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish. Ogawa Y, Shiraki T, Kojima D, Fukada Y. Proc Biol Sci. 2015 Aug 7;282(1812). pii: 20150659. doi: 10.1098/rspb.2015.0659. PubMed |
| alpha-5 Laminin Synthesized by Human Pluripotent Stem Cells Promotes Self-Renewal. Laperle A, Hsiao C, Lampe M, Mortier J, Saha K, Palecek SP, Masters KS. Stem Cell Reports. 2015 Aug 11;5(2):195-206. doi: 10.1016/j.stemcr.2015.06.009. Epub 2015 Jul 30. PubMed |
| TALEN- and CRISPR/Cas9-Mediated Gene Editing in Human Pluripotent Stem Cells Using Lipid-Based Transfection. Hendriks WT, Jiang X, Daheron L, Cowan CA. Curr Protoc Stem Cell Biol. 2015 Aug 3;34:5B.3.1-5B.3.25. doi: 10.1002/9780470151808.sc05b03s34. PubMed |
| Transcriptomic Profiling and H3K27me3 Distribution Reveal Both Demethylase-Dependent and Independent Regulation of Developmental Gene Transcription in Cell Differentiation. Kang SC, Kim SK, Chai JC, Kim SH, Won KJ, Lee YS, Jung KH, Chai YG. PLoS One. 2015 Aug 11;10(8):e0135276. doi: 10.1371/journal.pone.0135276. eCollection 2015. PubMed |
| Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids. Freedman BS, Brooks CR, Lam AQ, Fu H, Morizane R, Agrawal V, Saad AF, Li MK, Hughes MR, Werff RV, Peters DT, Lu J, Baccei A, Siedlecki AM, Valerius MT, Musunuru K, McNagny KM, Steinman TI, Zhou J, Lerou PH, Bonventre JV. Nat Commun. 2015 Oct 23;6:8715. doi: 10.1038/ncomms9715. PubMed |
| High-throughput genotyping of CRISPR/Cas9-mediated mutants using fluorescent PCR-capillary gel electrophoresis. Ramlee MK, Yan T, Cheung AM, Chuah CT, Li S. Sci Rep. 2015 Oct 26;5:15587. doi: 10.1038/srep15587. PubMed |
| Targeted Integration of Single-Copy Transgenes in Drosophila melanogaster Tissue-Culture Cells Using Recombination-Mediated Cassette Exchange. Manivannan SN, Jacobsen TL, Lyon P, Selvaraj B, Halpin P, Simcox A. Genetics. 2015 Dec;201(4):1319-28. doi: 10.1534/genetics.115.181230. Epub 2015 Oct 23. PubMed |
| Genome-Edited Human Pluripotent Stem Cell-Derived Macrophages as a Model of Reverse Cholesterol Transport--Brief Report. Gupta RM, Meissner TB, Cowan CA, Musunuru K. Arterioscler Thromb Vasc Biol. 2016 Jan;36(1):15-8. doi: 10.1161/ATVBAHA.115.305956. Epub 2015 Nov 5. PubMed |
| Effects on the transcriptome upon deletion of a distal element cannot be predicted by the size of the H3K27Ac peak in human cells. Tak YG, Hung Y, Yao L, Grimmer MR, Do A, Bhakta MS, O'Geen H, Segal DJ, Farnham PJ. Nucleic Acids Res. 2016 Jan 6. pii: gkv1530. PubMed |
ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.
Yoshimi K, Kunihiro Y, Kaneko T, Nagahora H, Voigt B, Mashimo T.
Nat Commun. 2016 Jan 20;7:10431. doi: 10.1038/ncomms10431.
PubMed
Associated Plasmids |
| Smad2 and Smad3 have differential sensitivity in relaying TGFbeta signaling and inversely regulate early lineage specification. Liu L, Liu X, Ren X, Tian Y, Chen Z, Xu X, Du Y, Jiang C, Fang Y, Liu Z, Fan B, Zhang Q, Jin G, Yang X, Zhang X. Sci Rep. 2016 Feb 24;6:21602. doi: 10.1038/srep21602. PubMed |
2C-Cas9: a versatile tool for clonal analysis of gene function.
Di Donato V, De Santis F, Auer TO, Testa N, Sanchez-Iranzo H, Mercader N, Concordet JP, Del Bene F.
Genome Res. 2016 Mar 8.
PubMed
Associated Plasmids |
| A Method for Genome Editing in Human Pluripotent Stem Cells. Smith C, Ye Z, Cheng L. Cold Spring Harb Protoc. 2016 Apr 1;2016(4):pdb.prot090217. doi: 10.1101/pdb.prot090217. PubMed |
| Genome Editing in Human Pluripotent Stem Cells. Smith C, Ye Z, Cheng L. Cold Spring Harb Protoc. 2016 Apr 1;2016(4):pdb.top086819. doi: 10.1101/pdb.top086819. PubMed |
| Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells. Moorthy SD, Mitchell JA. J Vis Exp. 2016 Apr 2;(110). doi: 10.3791/53552. PubMed |
| Site-specific genome editing for correction of induced pluripotent stem cells derived from dominant dystrophic epidermolysis bullosa. Shinkuma S, Guo Z, Christiano AM. Proc Natl Acad Sci U S A. 2016 May 17;113(20):5676-81. doi: 10.1073/pnas.1512028113. Epub 2016 May 3. PubMed |
| Drug conjugated nanoparticles activated by cancer cell specific mRNA. Gossai NP, Naumann JA, Li NS, Zamora EA, Gordon DJ, Piccirilli JA, Gordon PM. Oncotarget. 2016 Jun 21;7(25):38243-38256. doi: 10.18632/oncotarget.9430. PubMed |
| Mitochondrial dysfunction remodels one-carbon metabolism in human cells. Bao XR, Ong SE, Goldberger O, Peng J, Sharma R, Thompson DA, Vafai SB, Cox AG, Marutani E, Ichinose F, Goessling W, Regev A, Carr SA, Clish CB, Mootha VK. Elife. 2016 Jun 16;5. pii: e10575. doi: 10.7554/eLife.10575. PubMed |
| Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells. Liu Z, Hui Y, Shi L, Chen Z, Xu X, Chi L, Fan B, Fang Y, Liu Y, Ma L, Wang Y, Xiao L, Zhang Q, Jin G, Liu L, Zhang X. Stem Cell Reports. 2016 Sep 13;7(3):496-507. doi: 10.1016/j.stemcr.2016.07.021. Epub 2016 Sep 1. PubMed |
| Direct Mapping of Additional Modifications on Phosphorylated O-glycans of alpha-Dystroglycan by Mass Spectrometry Analysis in Conjunction with Knocking Out of Causative Genes for Dystroglycanopathy. Yagi H, Kuo CW, Obayashi T, Ninagawa S, Khoo KH, Kato K. Mol Cell Proteomics. 2016 Nov;15(11):3424-3434. doi: 10.1074/mcp.M116.062729. Epub 2016 Sep 6. PubMed |
| The DPYSL2 gene connects mTOR and schizophrenia. Pham X, Song G, Lao S, Goff L, Zhu H, Valle D, Avramopoulos D. Transl Psychiatry. 2016 Nov 1;6(11):e933. doi: 10.1038/tp.2016.204. PubMed |
| Capturing the biology of disease severity in a PSC-based model of familial dysautonomia. Zeltner N, Fattahi F, Dubois NC, Saurat N, Lafaille F, Shang L, Zimmer B, Tchieu J, Soliman MA, Lee G, Casanova JL, Studer L. Nat Med. 2016 Dec;22(12):1421-1427. doi: 10.1038/nm.4220. Epub 2016 Nov 14. PubMed |
| Enhancers and super-enhancers have an equivalent regulatory role in embryonic stem cells through regulation of single or multiple genes. Moorthy SD, Davidson S, Shchuka VM, Singh G, Malek-Gilani N, Langroudi L, Martchenko A, So V, Macpherson NN, Mitchell JA. Genome Res. 2016 Nov 28. pii: gr.210930.116. PubMed |
| ZEB1 is neither sufficient nor required for epithelial-mesenchymal transition in LS174T colorectal cancer cells. Jagle S, Dertmann A, Schrempp M, Hecht A. Biochem Biophys Res Commun. 2017 Jan 22;482(4):1226-1232. doi: 10.1016/j.bbrc.2016.12.017. Epub 2016 Dec 5. PubMed |
| Deletion of the Polycomb-Group Protein EZH2 Leads to Compromised Self-Renewal and Differentiation Defects in Human Embryonic Stem Cells. Collinson A, Collier AJ, Morgan NP, Sienerth AR, Chandra T, Andrews S, Rugg-Gunn PJ. Cell Rep. 2016 Dec 6;17(10):2700-2714. doi: 10.1016/j.celrep.2016.11.032. PubMed |
| Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells. Bressan RB, Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S, Skarnes WC, Pollard SM. Development. 2017 Feb 15;144(4):635-648. doi: 10.1242/dev.140855. Epub 2017 Jan 17. PubMed |
| Precise and efficient scarless genome editing in stem cells using CORRECT. Kwart D, Paquet D, Teo S, Tessier-Lavigne M. Nat Protoc. 2017 Feb;12(2):329-354. doi: 10.1038/nprot.2016.171. Epub 2017 Jan 19. PubMed |
Re-engineered RNA-Guided FokI-Nucleases for Improved Genome Editing in Human Cells.
Havlicek S, Shen Y, Alpagu Y, Bruntraeger MB, Zufir NB, Phuah ZY, Fu Z, Dunn NR, Stanton LW.
Mol Ther. 2017 Feb 1;25(2):342-355. doi: 10.1016/j.ymthe.2016.11.007.
PubMed
Associated Plasmids |
| CREB Signaling Is Involved in Rett Syndrome Pathogenesis. Bu Q, Wang A, Hamzah H, Waldman A, Jiang K, Dong Q, Li R, Kim J, Turner D, Chang Q. J Neurosci. 2017 Mar 29;37(13):3671-3685. doi: 10.1523/JNEUROSCI.3735-16.2017. Epub 2017 Mar 7. PubMed |
| ORAI2 modulates store-operated calcium entry and T cell-mediated immunity. Vaeth M, Yang J, Yamashita M, Zee I, Eckstein M, Knosp C, Kaufmann U, Karoly Jani P, Lacruz RS, Flockerzi V, Kacskovics I, Prakriya M, Feske S. Nat Commun. 2017 Mar 15;8:14714. doi: 10.1038/ncomms14714. PubMed |
| Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. Balczon R, Morrow KA, Zhou C, Edmonds B, Alexeyev M, Pittet JF, Wagener BM, Moser SA, Leavesley S, Zha X, Frank DW, Stevens T. FASEB J. 2017 Jul;31(7):2785-2796. doi: 10.1096/fj.201601042RR. Epub 2017 Mar 17. PubMed |
| Genome Editing in Human Pluripotent Stem Cells. Carlson-Stevermer J, Saha K. Methods Mol Biol. 2017;1590:165-174. doi: 10.1007/978-1-4939-6921-0_12. PubMed |
| Large, Diverse Population Cohorts of hiPSCs and Derived Hepatocyte-like Cells Reveal Functional Genetic Variation at Blood Lipid-Associated Loci. Pashos EE, Park Y, Wang X, Raghavan A, Yang W, Abbey D, Peters DT, Arbelaez J, Hernandez M, Kuperwasser N, Li W, Lian Z, Liu Y, Lv W, Lytle-Gabbin SL, Marchadier DH, Rogov P, Shi J, Slovik KJ, Stylianou IM, Wang L, Yan R, Zhang X, Kathiresan S, Duncan SA, Mikkelsen TS, Morrisey EE, Rader DJ, Brown CD, Musunuru K. Cell Stem Cell. 2017 Apr 6;20(4):558-570.e10. doi: 10.1016/j.stem.2017.03.017. PubMed |
| Using CRISPR-Cas9 to Generate Gene-Corrected Autologous iPSCs for the Treatment of Inherited Retinal Degeneration. Burnight ER, Gupta M, Wiley LA, Anfinson KR, Tran A, Triboulet R, Hoffmann JM, Klaahsen DL, Andorf JL, Jiao C, Sohn EH, Adur MK, Ross JW, Mullins RF, Daley GQ, Schlaeger TM, Stone EM, Tucker BA. Mol Ther. 2017 Jun 12. pii: S1525-0016(17)30233-2. doi: 10.1016/j.ymthe.2017.05.015. PubMed |
| In Vivo Base Editing of PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) as a Therapeutic Alternative to Genome Editing. Chadwick AC, Wang X, Musunuru K. Arterioscler Thromb Vasc Biol. 2017 Sep;37(9):1741-1747. doi: 10.1161/ATVBAHA.117.309881. Epub 2017 Jul 27. PubMed |
| Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation. Thomas CA, Tejwani L, Trujillo CA, Negraes PD, Herai RH, Mesci P, Macia A, Crow YJ, Muotri AR. Cell Stem Cell. 2017 Sep 7;21(3):319-331.e8. doi: 10.1016/j.stem.2017.07.009. Epub 2017 Aug 10. PubMed |
| CRISPR/Cas9 and piggyBac-mediated footprint-free LRRK2-G2019S knock-in reveals neuronal complexity phenotypes and alpha-Synuclein modulation in dopaminergic neurons. Qing X, Walter J, Jarazo J, Arias-Fuenzalida J, Hillje AL, Schwamborn JC. Stem Cell Res. 2017 Oct;24:44-50. doi: 10.1016/j.scr.2017.08.013. Epub 2017 Aug 18. PubMed |
| Dynamics and regulation of nuclear import and nuclear movements of HIV-1 complexes. Burdick RC, Delviks-Frankenberry KA, Chen J, Janaka SK, Sastri J, Hu WS, Pathak VK. PLoS Pathog. 2017 Aug 21;13(8):e1006570. doi: 10.1371/journal.ppat.1006570. eCollection 2017 Aug. PubMed |
A Modular Platform for Differentiation of Human PSCs into All Major Ectodermal Lineages.
Tchieu J, Zimmer B, Fattahi F, Amin S, Zeltner N, Chen S, Studer L.
Cell Stem Cell. 2017 Sep 7;21(3):399-410.e7. doi: 10.1016/j.stem.2017.08.015.
PubMed
Associated Plasmids |
| Novel Function of a Transcription Factor WT1 in Regulating Decidualization in Human Endometrial Stromal Cells and Its Molecular Mechanism. Tamura I, Shirafuta Y, Jozaki K, Kajimura T, Shinagawa M, Maekawa R, Taketani T, Asada H, Sato S, Tamura H, Sugino N. Endocrinology. 2017 Oct 1;158(10):3696-3707. doi: 10.1210/en.2017-00478. PubMed |
| Multiplex CRISPR/Cas9-Based Genome Editing in Human Hematopoietic Stem Cells Models Clonal Hematopoiesis and Myeloid Neoplasia. Tothova Z, Krill-Burger JM, Popova KD, Landers CC, Sievers QL, Yudovich D, Belizaire R, Aster JC, Morgan EA, Tsherniak A, Ebert BL. Cell Stem Cell. 2017 Oct 5;21(4):547-555.e8. doi: 10.1016/j.stem.2017.07.015. PubMed |
| SNAIL1-mediated downregulation of FOXA proteins facilitates the inactivation of transcriptional enhancer elements at key epithelial genes in colorectal cancer cells. Jagle S, Busch H, Freihen V, Beyes S, Schrempp M, Boerries M, Hecht A. PLoS Genet. 2017 Nov 20;13(11):e1007109. doi: 10.1371/journal.pgen.1007109. eCollection 2017 Nov. PubMed |
| SN-38 Conjugated Gold Nanoparticles Activated by Ewing Sarcoma Specific mRNAs Exhibit In Vitro and In Vivo Efficacy. Naumann JA, Widen JC, Jonart LA, Ebadi M, Tang J, Gordon DJ, Harki DA, Gordon PM. Bioconjug Chem. 2018 Apr 18;29(4):1111-1118. doi: 10.1021/acs.bioconjchem.7b00774. Epub 2018 Feb 19. PubMed |
| The distal upstream region of insulin-like growth factor-binding protein-1 enhances its expression in endometrial stromal cells during decidualization. Tamura I, Jozaki K, Sato S, Shirafuta Y, Shinagawa M, Maekawa R, Taketani T, Asada H, Tamura H, Sugino N. J Biol Chem. 2018 Apr 6;293(14):5270-5280. doi: 10.1074/jbc.RA117.000234. Epub 2018 Feb 16. PubMed |
| Dissecting the Causal Mechanism of X-Linked Dystonia-Parkinsonism by Integrating Genome and Transcriptome Assembly. Aneichyk T, Hendriks WT, Yadav R, Shin D, Gao D, Vaine CA, Collins RL, Domingo A, Currall B, Stortchevoi A, Multhaupt-Buell T, Penney EB, Cruz L, Dhakal J, Brand H, Hanscom C, Antolik C, Dy M, Ragavendran A, Underwood J, Cantsilieris S, Munson KM, Eichler EE, Acuna P, Go C, Jamora RDG, Rosales RL, Church DM, Williams SR, Garcia S, Klein C, Muller U, Wilhelmsen KC, Timmers HTM, Sapir Y, Wainger BJ, Henderson D, Ito N, Weisenfeld N, Jaffe D, Sharma N, Breakefield XO, Ozelius LJ, Bragg DC, Talkowski ME. Cell. 2018 Feb 22;172(5):897-909.e21. doi: 10.1016/j.cell.2018.02.011. PubMed |
Conditional gene knockout and reconstitution in human iPSCs with an inducible Cas9 system.
Wu M, Liu S, Gao Y, Bai H, Machairaki V, Li G, Chen T, Cheng L.
Stem Cell Res. 2018 May;29:6-14. doi: 10.1016/j.scr.2018.03.003. Epub 2018 Mar 10.
PubMed
Associated Plasmids |
| Genetic identification of leptin neural circuits in energy and glucose homeostases. Xu J, Bartolome CL, Low CS, Yi X, Chien CH, Wang P, Kong D. Nature. 2018 Apr;556(7702):505-509. doi: 10.1038/s41586-018-0049-7. Epub 2018 Apr 18. PubMed |
| The activation of STIM1 mediates S-phase arrest and cell death in paraquat induced acute lung intoxication. Fan H, Huang H, Hu L, Zhu W, Yu Y, Lou J, Hu L, Chen F. Toxicol Lett. 2018 Aug;292:123-135. doi: 10.1016/j.toxlet.2018.04.030. Epub 2018 Apr 27. PubMed |
| Large-Scale Expansion of Human iPSC-Derived Skeletal Muscle Cells for Disease Modeling and Cell-Based Therapeutic Strategies. van der Wal E, Herrero-Hernandez P, Wan R, Broeders M, In 't Groen SLM, van Gestel TJM, van IJcken WFJ, Cheung TH, van der Ploeg AT, Schaaf GJ, Pijnappel WWMP. Stem Cell Reports. 2018 Jun 5;10(6):1975-1990. doi: 10.1016/j.stemcr.2018.04.002. Epub 2018 May 3. PubMed |
| PRDM1 silences stem cell-related genes and inhibits proliferation of human colon tumor organoids. Liu C, Banister CE, Weige CC, Altomare D, Richardson JH, Contreras CM, Buckhaults PJ. Proc Natl Acad Sci U S A. 2018 May 29;115(22):E5066-E5075. doi: 10.1073/pnas.1802902115. Epub 2018 May 14. PubMed |
| DMSO increases efficiency of genome editing at two non-coding loci. Stratigopoulos G, De Rosa MC, LeDuc CA, Leibel RL, Doege CA. PLoS One. 2018 Jun 4;13(6):e0198637. doi: 10.1371/journal.pone.0198637. eCollection 2018. PubMed |
| Accounting for tumor heterogeneity when using CRISPR-Cas9 for cancer progression and drug sensitivity studies. Olive JF, Qin Y, DeCristo MJ, Laszewski T, Greathouse F, McAllister SS. PLoS One. 2018 Jun 13;13(6):e0198790. doi: 10.1371/journal.pone.0198790. eCollection 2018. PubMed |
| Straightforward Delivery of Linearized Double-Stranded DNA Encoding sgRNA and Donor DNA for the Generation of Single Nucleotide Variants Based on the CRISPR/Cas9 System. Jun S, Lim H, Jang H, Lee W, Ahn J, Lee JH, Bang D. ACS Synth Biol. 2018 Jul 20;7(7):1651-1659. doi: 10.1021/acssynbio.7b00345. Epub 2018 Jul 2. PubMed |
| The E3 ubiquitin ligase SMURF1 regulates cell-fate specification and outflow tract septation during mammalian heart development. Koefoed K, Skat-Rordam J, Andersen P, Warzecha CB, Pye M, Andersen TA, Ajbro KD, Bendsen E, Narimatsu M, Vilhardt F, Pedersen LB, Wrana JL, Anderson RH, Mollgard K, Christensen ST, Larsen LA. Sci Rep. 2018 Jun 22;8(1):9542. doi: 10.1038/s41598-018-27854-8. PubMed |
| Tissue-specific expression and post-transcriptional regulation of the ATPase inhibitory factor 1 (IF1) in human and mouse tissues. Esparza-Molto PB, Nuevo-Tapioles C, Chamorro M, Najera L, Torresano L, Santacatterina F, Cuezva JM. FASEB J. 2019 Feb;33(2):1836-1851. doi: 10.1096/fj.201800756R. Epub 2018 Sep 11. PubMed |
| Esrrb extinction triggers dismantling of naive pluripotency and marks commitment to differentiation. Festuccia N, Halbritter F, Corsinotti A, Gagliardi A, Colby D, Tomlinson SR, Chambers I. EMBO J. 2018 Nov 2;37(21). pii: embj.201695476. doi: 10.15252/embj.201695476. Epub 2018 Oct 1. PubMed |
| In utero CRISPR-mediated therapeutic editing of metabolic genes. Rossidis AC, Stratigis JD, Chadwick AC, Hartman HA, Ahn NJ, Li H, Singh K, Coons BE, Li L, Lv W, Zoltick PW, Alapati D, Zacharias W, Jain R, Morrisey EE, Musunuru K, Peranteau WH. Nat Med. 2018 Oct;24(10):1513-1518. doi: 10.1038/s41591-018-0184-6. Epub 2018 Oct 8. PubMed |
| Highly Efficient CRISPR-Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells. Maguire JA, Cardenas-Diaz FL, Gadue P, French DL. Curr Protoc Stem Cell Biol. 2019 Feb;48(1):e64. doi: 10.1002/cpsc.64. Epub 2018 Oct 24. PubMed |
| Patient Adipose Stem Cell-Derived Adipocytes Reveal Genetic Variation that Predicts Antidiabetic Drug Response. Hu W, Jiang C, Guan D, Dierickx P, Zhang R, Moscati A, Nadkarni GN, Steger DJ, Loos RJF, Hu C, Jia W, Soccio RE, Lazar MA. Cell Stem Cell. 2019 Feb 7;24(2):299-308.e6. doi: 10.1016/j.stem.2018.11.018. Epub 2019 Jan 10. PubMed |
| ATL3 gene mutation in a Chinese family with hereditary sensory neuropathy type 1F. Xu H, Zhang C, Cao L, Song J, Xu X, Zhang B, Chen B, Zhao G. J Peripher Nerv Syst. 2019 Mar;24(1):150-155. doi: 10.1111/jns.12309. Epub 2019 Feb 21. PubMed |
| Metabolic and Organelle Morphology Defects in Mice and Human Patients Define Spinocerebellar Ataxia Type 7 as a Mitochondrial Disease. Ward JM, Stoyas CA, Switonski PM, Ichou F, Fan W, Collins B, Wall CE, Adanyeguh I, Niu C, Sopher BL, Kinoshita C, Morrison RS, Durr A, Muotri AR, Evans RM, Mochel F, La Spada AR. Cell Rep. 2019 Jan 29;26(5):1189-1202.e6. doi: 10.1016/j.celrep.2019.01.028. PubMed |
| High-Dose Paraquat Induces Human Bronchial 16HBE Cell Death and Aggravates Acute Lung Intoxication in Mice by Regulating Keap1/p65/Nrf2 Signal Pathway. Yao J, Zhang J, Tai W, Deng S, Li T, Wu W, Pu L, Fan D, Lei W, Zhang T, Dong Z. Inflammation. 2019 Apr;42(2):471-484. doi: 10.1007/s10753-018-00956-1. PubMed |
| Efficient Gene Reframing Therapy for Recessive Dystrophic Epidermolysis Bullosa with CRISPR/Cas9. Takashima S, Shinkuma S, Fujita Y, Nomura T, Ujiie H, Natsuga K, Iwata H, Nakamura H, Vorobyev A, Abe R, Shimizu H. J Invest Dermatol. 2019 Aug;139(8):1711-1721.e4. doi: 10.1016/j.jid.2019.02.015. Epub 2019 Mar 1. PubMed |
| High-glucose induces retinal pigment epithelium mitochondrial pathways of apoptosis and inhibits mitophagy by regulating ROS/PINK1/Parkin signal pathway. Zhang Y, Xi X, Mei Y, Zhao X, Zhou L, Ma M, Liu S, Zha X, Yang Y. Biomed Pharmacother. 2019 Mar;111:1315-1325. doi: 10.1016/j.biopha.2019.01.034. Epub 2019 Jan 15. PubMed |
| Spindle Assembly Checkpoint Inhibition Can Resensitize p53-Null Stem Cells to Cancer Chemotherapy. Liu C, Banister CE, Buckhaults PJ. Cancer Res. 2019 May 1;79(9):2392-2403. doi: 10.1158/0008-5472.CAN-18-3024. Epub 2019 Mar 12. PubMed |
| Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5(-/-) mice. Li Y, Tinoco R, Elmen L, Segota I, Xian Y, Fujita Y, Sahu A, Zarecki R, Marie K, Feng Y, Khateb A, Frederick DT, Ashkenazi SK, Kim H, Perez EG, Day CP, Segura Munoz RS, Schmaltz R, Yooseph S, Tam MA, Zhang T, Avitan-Hersh E, Tzur L, Roizman S, Boyango I, Bar-Sela G, Orian A, Kaufman RJ, Bosenberg M, Goding CR, Baaten B, Levesque MP, Dummer R, Brown K, Merlino G, Ruppin E, Flaherty K, Ramer-Tait A, Long T, Peterson SN, Bradley LM, Ronai ZA. Nat Commun. 2019 Apr 2;10(1):1492. doi: 10.1038/s41467-019-09525-y. PubMed |
| Protection of ZIKV infection-induced neuropathy by abrogation of acute antiviral response in human neural progenitors. Liu L, Chen Z, Zhang X, Li S, Hui Y, Feng H, Du Y, Jin G, Zhou X, Zhang X. Cell Death Differ. 2019 Apr 5. pii: 10.1038/s41418-019-0324-7. doi: 10.1038/s41418-019-0324-7. PubMed |
| Inactivation of CFTR by CRISPR/Cas9 alters transcriptional regulation of inflammatory pathways and other networks. Hao S, Roesch EA, Perez A, Weiner RL, Henderson LC, Cummings L, Consiglio P, Pajka J, Eisenberg A, Yeh L, Cotton CU, Drumm ML. J Cyst Fibros. 2019 May 21. pii: S1569-1993(19)30105-5. doi: 10.1016/j.jcf.2019.05.003. PubMed |
| Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids. Ouchi R, Togo S, Kimura M, Shinozawa T, Koido M, Koike H, Thompson W, Karns RA, Mayhew CN, McGrath PS, McCauley HA, Zhang RR, Lewis K, Hakozaki S, Ferguson A, Saiki N, Yoneyama Y, Takeuchi I, Mabuchi Y, Akazawa C, Yoshikawa HY, Wells JM, Takebe T. Cell Metab. 2019 May 30. pii: S1550-4131(19)30247-5. doi: 10.1016/j.cmet.2019.05.007. PubMed |
| Indirect activation of constitutive androstane receptor in three-dimensionally cultured HepG2 cells. Yokobori K, Azuma I, Chiba K, Akita H, Furihata T, Kobayashi K. Biochem Pharmacol. 2019 Jun 13;168:26-37. doi: 10.1016/j.bcp.2019.06.011. PubMed |
KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control.
Dhaliwal NK, Abatti LE, Mitchell JA.
Genes Dev. 2019 Jun 20. pii: gad.324319.119. doi: 10.1101/gad.324319.119.
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| Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. Low JH, Li P, Chew EGY, Zhou B, Suzuki K, Zhang T, Lian MM, Liu M, Aizawa E, Rodriguez Esteban C, Yong KSM, Chen Q, Campistol JM, Fang M, Khor CC, Foo JN, Izpisua Belmonte JC, Xia Y. Cell Stem Cell. 2019 Sep 5;25(3):373-387.e9. doi: 10.1016/j.stem.2019.06.009. Epub 2019 Jul 11. PubMed |
| Magnesium transporter 1 (MAGT1) deficiency causes selective defects in N-linked glycosylation and expression of immune-response genes. Matsuda-Lennikov M, Biancalana M, Zou J, Ravell JC, Zheng L, Kanellopoulou C, Jiang P, Notarangelo G, Jing H, Masutani E, Oler AJ, Olano LR, Schulz BL, Lenardo MJ. J Biol Chem. 2019 Sep 13;294(37):13638-13656. doi: 10.1074/jbc.RA119.008903. Epub 2019 Jul 23. PubMed |
| Genome-wide mapping of DNA-binding sites identifies stemness-related genes as directly repressed targets of SNAIL1 in colorectal cancer cells. Beyes S, Andrieux G, Schrempp M, Aicher D, Wenzel J, Anton-Garcia P, Boerries M, Hecht A. Oncogene. 2019 Oct;38(40):6647-6661. doi: 10.1038/s41388-019-0905-4. Epub 2019 Aug 7. PubMed |
| A Large Panel of Isogenic APP and PSEN1 Mutant Human iPSC Neurons Reveals Shared Endosomal Abnormalities Mediated by APP beta-CTFs, Not Abeta. Kwart D, Gregg A, Scheckel C, Murphy EA, Paquet D, Duffield M, Fak J, Olsen O, Darnell RB, Tessier-Lavigne M. Neuron. 2019 Oct 23;104(2):256-270.e5. doi: 10.1016/j.neuron.2019.07.010. Epub 2019 Aug 12. PubMed |
| Structural interaction between DISC1 and ATF4 underlying transcriptional and synaptic dysregulation in an iPSC model of mental disorders. Wang X, Ye F, Wen Z, Guo Z, Yu C, Huang WK, Rojas Ringeling F, Su Y, Zheng W, Zhou G, Christian KM, Song H, Zhang M, Ming GL. Mol Psychiatry. 2019 Aug 23. pii: 10.1038/s41380-019-0485-2. doi: 10.1038/s41380-019-0485-2. PubMed |
| SNAIL1 employs beta-Catenin-LEF1 complexes to control colorectal cancer cell invasion and proliferation. Freihen V, Ronsch K, Mastroianni J, Frey P, Rose K, Boerries M, Zeiser R, Busch H, Hecht A. Int J Cancer. 2020 Apr 15;146(8):2229-2242. doi: 10.1002/ijc.32644. Epub 2019 Sep 18. PubMed |
| Murine Perinatal beta-Cell Proliferation and the Differentiation of Human Stem Cell-Derived Insulin-Expressing Cells Require NEUROD1. Romer AI, Singer RA, Sui L, Egli D, Sussel L. Diabetes. 2019 Dec;68(12):2259-2271. doi: 10.2337/db19-0117. Epub 2019 Sep 13. PubMed |
| Modelling human hepato-biliary-pancreatic organogenesis from the foregut-midgut boundary. Koike H, Iwasawa K, Ouchi R, Maezawa M, Giesbrecht K, Saiki N, Ferguson A, Kimura M, Thompson WL, Wells JM, Zorn AM, Takebe T. Nature. 2019 Oct;574(7776):112-116. doi: 10.1038/s41586-019-1598-0. Epub 2019 Sep 25. PubMed |
| Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells. Cardenas-Diaz FL, Maguire JA, Gadue P, French DL. J Vis Exp. 2019 Sep 25;(151). doi: 10.3791/60085. PubMed |
| NFAT primes the human RORC locus for RORgammat expression in CD4(+) T cells. Yahia-Cherbal H, Rybczynska M, Lovecchio D, Stephen T, Lescale C, Placek K, Larghero J, Rogge L, Bianchi E. Nat Commun. 2019 Oct 16;10(1):4698. doi: 10.1038/s41467-019-12680-x. PubMed |
| Efficient gene correction of an aberrant splice site in beta-thalassaemia iPSCs by CRISPR/Cas9 and single-strand oligodeoxynucleotides. Xiong Z, Xie Y, Yang Y, Xue Y, Wang D, Lin S, Chen D, Lu D, He L, Song B, Yang Y, Sun X. J Cell Mol Med. 2019 Dec;23(12):8046-8057. doi: 10.1111/jcmm.14669. Epub 2019 Oct 21. PubMed |
| Dysregulation of STAT3 signaling is associated with endplate-oriented herniations of the intervertebral disc in Adgrg6 mutant mice. Liu Z, Easson GWD, Zhao J, Makki N, Ahituv N, Hilton MJ, Tang SY, Gray RS. PLoS Genet. 2019 Oct 25;15(10):e1008096. doi: 10.1371/journal.pgen.1008096. eCollection 2019 Oct. PubMed |
| Regulation of CHD2 expression by the Chaserr long noncoding RNA gene is essential for viability. Rom A, Melamed L, Gil N, Goldrich MJ, Kadir R, Golan M, Biton I, Perry RB, Ulitsky I. Nat Commun. 2019 Nov 8;10(1):5092. doi: 10.1038/s41467-019-13075-8. PubMed |
| Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus. Goldstein JM, Valido A, Lewandowski JP, Walker RG, Mills MJ, Messemer KA, Besseling P, Lee KH, Wattrus SJ, Cho M, Lee RT, Wagers AJ. Sci Rep. 2019 Dec 9;9(1):18613. doi: 10.1038/s41598-019-54766-y. PubMed |
| Dectin-1 Binding to Annexins on Apoptotic Cells Induces Peripheral Immune Tolerance via NADPH Oxidase-2. Bode K, Bujupi F, Link C, Hein T, Zimmermann S, Peiris D, Jaquet V, Lepenies B, Weyd H, Krammer PH. Cell Rep. 2019 Dec 24;29(13):4435-4446.e9. doi: 10.1016/j.celrep.2019.11.086. PubMed |
| Effects of MYBPC3 loss-of-function mutations preceding hypertrophic cardiomyopathy. Helms AS, Tang VT, O'Leary TS, Friedline S, Wauchope M, Arora A, Wasserman AH, Smith ED, Lee LM, Wen XW, Shavit JA, Liu AP, Previs MJ, Day SM. JCI Insight. 2020 Jan 30;5(2). pii: 133782. doi: 10.1172/jci.insight.133782. PubMed |
| TGF-beta-Induced TMEPAI Attenuates the Response of Triple-Negative Breast Cancer Cells to Doxorubicin and Paclitaxel. Wardhani BW, Puteri MU, Watanabe Y, Louisa M, Setiabudy R, Kato M. J Exp Pharmacol. 2020 Jan 23;12:17-26. doi: 10.2147/JEP.S235233. eCollection 2020. PubMed |
| Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor. Yagi H, Yagi-Utsumi M, Honda R, Ohta Y, Saito T, Nishio M, Ninagawa S, Suzuki K, Anzai T, Kamiya Y, Aoki K, Nakanishi M, Satoh T, Kato K. Nat Commun. 2020 Mar 13;11(1):1368. doi: 10.1038/s41467-020-15192-1. PubMed |
Fluorescent indicators for continuous and lineage-specific reporting of cell-cycle phases in human pluripotent stem cells.
Chang Y, Hellwarth PB, Randolph LN, Sun Y, Xing Y, Zhu W, Lian XL, Bao X.
Biotechnol Bioeng. 2020 Apr 11. doi: 10.1002/bit.27352.
PubMed
Associated Plasmids |
Enabling large-scale genome editing at repetitive elements by reducing DNA nicking.
Smith CJ, Castanon O, Said K, Volf V, Khoshakhlagh P, Hornick A, Ferreira R, Wu CT, Guell M, Garg S, Ng AHM, Myllykallio H, Church GM.
Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239.
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| Patient hiPSCs Identify Vascular Smooth Muscle Arylacetamide Deacetylase as Protective against Atherosclerosis. Toyohara T, Roudnicky F, Florido MHC, Nakano T, Yu H, Katsuki S, Lee M, Meissner T, Friesen M, Davidow LS, Ptaszek L, Abe T, Rubin LL, Pereira AC, Aikawa M, Cowan CA. Cell Stem Cell. 2020 Jul 2;27(1):147-157.e7. doi: 10.1016/j.stem.2020.04.018. Epub 2020 May 14. PubMed |
| Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer. Das S, Chandrasekaran AP, Suresh B, Haq S, Kang JH, Lee SJ, Kim J, Kim J, Lee S, Kim HH, Kim KS, Ramakrishna S. Cell Death Differ. 2020 May 15. pii: 10.1038/s41418-020-0557-5. doi: 10.1038/s41418-020-0557-5. PubMed |
| Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing. Weisheit I, Kroeger JA, Malik R, Klimmt J, Crusius D, Dannert A, Dichgans M, Paquet D. Cell Rep. 2020 May 26;31(8):107689. doi: 10.1016/j.celrep.2020.107689. PubMed |
| A caveolin binding motif in Na/K-ATPase is required for stem cell differentiation and organogenesis in mammals and C. elegans. Wang X, Cai L, Xie JX, Cui X, Zhang J, Wang J, Chen Y, Larre I, Shapiro JI, Pierre SV, Wu D, Zhu GZ, Xie Z. Sci Adv. 2020 May 27;6(22):eaaw5851. doi: 10.1126/sciadv.aaw5851. eCollection 2020 May. PubMed |
| KLF4 Acts as a wt-CFTR Suppressor through an AKT-Mediated Pathway. Sousa L, Pankonien I, Clarke LA, Silva I, Kunzelmann K, Amaral MD. Cells. 2020 Jul 2;9(7). pii: cells9071607. doi: 10.3390/cells9071607. PubMed |
| In Vivo Chimeric Alzheimer's Disease Modeling of Apolipoprotein E4 Toxicity in Human Neurons. Najm R, Zalocusky KA, Zilberter M, Yoon SY, Hao Y, Koutsodendris N, Nelson M, Rao A, Taubes A, Jones EA, Huang Y. Cell Rep. 2020 Jul 28;32(4):107962. doi: 10.1016/j.celrep.2020.107962. PubMed |
Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function.
Xiong M, Tao Y, Gao Q, Feng B, Yan W, Zhou Y, Kotsonis TA, Yuan T, You Z, Wu Z, Xi J, Haberman A, Graham J, Block J, Zhou W, Chen Y, Zhang SC.
Cell Stem Cell. 2020 Sep 16. pii: S1934-5909(20)30410-0. doi: 10.1016/j.stem.2020.08.014.
PubMed
Associated Plasmids |
| Regulation of neuronal commitment in mouse embryonic stem cells by the Reno1/Bahcc1 locus. Hezroni H, Ben-Tov Perry R, Gil N, Degani N, Ulitsky I. EMBO Rep. 2020 Nov 5;21(11):e51264. doi: 10.15252/embr.202051264. Epub 2020 Sep 24. PubMed |
| Mitochondrial and Redox Modifications in Huntington Disease Induced Pluripotent Stem Cells Rescued by CRISPR/Cas9 CAGs Targeting. Lopes C, Tang Y, Anjo SI, Manadas B, Onofre I, de Almeida LP, Daley GQ, Schlaeger TM, Rego ACC. Front Cell Dev Biol. 2020 Sep 22;8:576592. doi: 10.3389/fcell.2020.576592. eCollection 2020. PubMed |
| Pathogenic LMNA variants disrupt cardiac lamina-chromatin interactions and de-repress alternative fate genes. Shah PP, Lv W, Rhoades JH, Poleshko A, Abbey D, Caporizzo MA, Linares-Saldana R, Heffler JG, Sayed N, Thomas D, Wang Q, Stanton LJ, Bedi K, Morley MP, Cappola TP, Owens AT, Margulies KB, Frank DB, Wu JC, Rader DJ, Yang W, Prosser BL, Musunuru K, Jain R. Cell Stem Cell. 2021 May 6;28(5):938-954.e9. doi: 10.1016/j.stem.2020.12.016. Epub 2021 Feb 1. PubMed |
| Bardet-Biedl syndrome proteins regulate intracellular signaling and neuronal function in patient-specific iPSC-derived neurons. Wang L, Liu Y, Stratigopoulos G, Panigrahi S, Sui L, Zhang Y, Leduc CA, Glover HJ, De Rosa MC, Burnett LC, Williams DJ, Shang L, Goland R, Tsang SH, Wardlaw S, Egli D, Zheng D, Doege CA, Leibel RL. J Clin Invest. 2021 Apr 15;131(8). pii: 146287. doi: 10.1172/JCI146287. PubMed |
| A flexible repertoire of transcription factor binding sites and a diversity threshold determines enhancer activity in embryonic stem cells. Singh G, Mullany S, Moorthy SD, Zhang R, Mehdi T, Tian R, Duncan AG, Moses AM, Mitchell JA. Genome Res. 2021 Apr;31(4):564-575. doi: 10.1101/gr.272468.120. Epub 2021 Mar 12. PubMed |
| Functional and Adaptive Significance of Promoter Mutations That Affect Divergent Myocardial Expressions of TRIM72 in Primates. Feng Y, Xu H, Liu J, Xie N, Gao L, He Y, Yao Y, Lv F, Zhang Y, Lu J, Zhang W, Li CY, Hu X, Yang Z, Xiao RP. Mol Biol Evol. 2021 Jun 25;38(7):2930-2945. doi: 10.1093/molbev/msab083. PubMed |
Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cells.
Breunig M, Merkle J, Wagner M, Melzer MK, Barth TFE, Engleitner T, Krumm J, Wiedenmann S, Cohrs CM, Perkhofer L, Jain G, Kruger J, Hermann PC, Schmid M, Madacsy T, Varga A, Griger J, Azoitei N, Muller M, Wessely O, Robey PG, Heller S, Dantes Z, Reichert M, Gunes C, Bolenz C, Kuhn F, Maleth J, Speier S, Liebau S, Sipos B, Kuster B, Seufferlein T, Rad R, Meier M, Hohwieler M, Kleger A.
Cell Stem Cell. 2021 Jun 3;28(6):1105-1124.e19. doi: 10.1016/j.stem.2021.03.005. Epub 2021 Apr 28.
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Associated Plasmids |
| Ubiquitin-Specific Protease 3 Deubiquitinates and Stabilizes Oct4 Protein in Human Embryonic Stem Cells. Rhie BH, Antao AM, Karapurkar JK, Kim MS, Jo WJ, Ramakrishna S, Kim KS. Int J Mol Sci. 2021 May 25;22(11). pii: ijms22115584. doi: 10.3390/ijms22115584. PubMed |
| Protocol for genome-scale CRISPR screening in engineered lineage reporter hPSCs to study cell fate determination. Zhou Z, Ma L, Zhang X. STAR Protoc. 2021 May 20;2(2):100548. doi: 10.1016/j.xpro.2021.100548. eCollection 2021 Jun 18. PubMed |
| Unraveling Ewing sarcoma tumorigenesis originating from patient-derived Mesenchymal Stem Cells. Sole A, Grossetete S, Heintze M, Babin L, Zaidi S, Revy P, Renouf B, De Cian A, Giovannangeli C, Pierre-Eugene C, Janoueix-Lerosey I, Couronne L, Kaltenbach S, Tomishima M, Jasin M, Grunewald TGP, Delattre O, Surdez D, Brunet E. Cancer Res. 2021 Aug 2. pii: 0008-5472.CAN-20-3837. doi: 10.1158/0008-5472.CAN-20-3837. PubMed |
| Human sensorimotor organoids derived from healthy and amyotrophic lateral sclerosis stem cells form neuromuscular junctions. Pereira JD, DuBreuil DM, Devlin AC, Held A, Sapir Y, Berezovski E, Hawrot J, Dorfman K, Chander V, Wainger BJ. Nat Commun. 2021 Aug 6;12(1):4744. doi: 10.1038/s41467-021-24776-4. PubMed |
| Actinin BioID reveals sarcomere crosstalk with oxidative metabolism through interactions with IGF2BP2. Ladha FA, Thakar K, Pettinato AM, Legere N, Ghahremani S, Cohn R, Romano R, Meredith E, Chen YS, Hinson JT. Cell Rep. 2021 Aug 10;36(6):109512. doi: 10.1016/j.celrep.2021.109512. PubMed |
| Mapping the evolving landscape of super-enhancers during cell differentiation. Kai Y, Li BE, Zhu M, Li GY, Chen F, Han Y, Cha HJ, Orkin SH, Cai W, Huang J, Yuan GC. Genome Biol. 2021 Sep 15;22(1):269. doi: 10.1186/s13059-021-02485-x. PubMed |
| Mutations and variants of ONECUT1 in diabetes. Philippi A, Heller S, Costa IG, Senee V, Breunig M, Li Z, Kwon G, Russell R, Illing A, Lin Q, Hohwieler M, Degavre A, Zalloua P, Liebau S, Schuster M, Krumm J, Zhang X, Geusz R, Benthuysen JR, Wang A, Chiou J, Gaulton K, Neubauer H, Simon E, Klein T, Wagner M, Nair G, Besse C, Dandine-Roulland C, Olaso R, Deleuze JF, Kuster B, Hebrok M, Seufferlein T, Sander M, Boehm BO, Oswald F, Nicolino M, Julier C, Kleger A. Nat Med. 2021 Nov;27(11):1928-1940. doi: 10.1038/s41591-021-01502-7. Epub 2021 Oct 18. PubMed |
| Extracellular vesicles released by human retinal pigment epithelium mediate increased polarised secretion of drusen proteins in response to AMD stressors. Flores-Bellver M, Mighty J, Aparicio-Domingo S, Li KV, Shi C, Zhou J, Cobb H, McGrath P, Michelis G, Lenhart P, Bilousova G, Heissel S, Rudy MJ, Coughlan C, Goodspeed AE, Becerra SP, Redenti S, Canto-Soler MV. J Extracell Vesicles. 2021 Nov;10(13):e12165. doi: 10.1002/jev2.12165. PubMed |
| Antagonistic Roles of the Tumor Suppressor miR-210-3p and Oncomucin MUC4 Forming a Negative Feedback Loop in Pancreatic Adenocarcinoma. Boukrout N, Souidi M, Lahdaoui F, Duchene B, Neve B, Coppin L, Leteurtre E, Torrisani J, Van Seuningen I, Jonckheere N. Cancers (Basel). 2021 Dec 9;13(24). pii: cancers13246197. doi: 10.3390/cancers13246197. PubMed |
| Genome-Wide CRISPR/Cas9-Based Screening for Deubiquitinase Subfamily Identifies Ubiquitin-Specific Protease 11 as a Novel Regulator of Osteogenic Differentiation. Kaushal K, Tyagi A, Karapurkar JK, Kim EJ, Tanguturi P, Kim KS, Jung HS, Ramakrishna S. Int J Mol Sci. 2022 Jan 13;23(2). pii: ijms23020856. doi: 10.3390/ijms23020856. PubMed |
| Genome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis. Kaushal K, Kim EJ, Tyagi A, Karapurkar JK, Haq S, Jung HS, Kim KS, Ramakrishna S. Cell Death Differ. 2022 Sep;29(9):1689-1704. doi: 10.1038/s41418-022-00956-7. Epub 2022 Mar 10. PubMed |
Optogenetic Control of Engrafted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Live Mice: A Proof-of-Concept Study.
Joshi J, Xu B, Rubart M, Chang Y, Bao X, Chaliki HP, Scott LR, Zhu W.
Cells. 2022 Mar 10;11(6). pii: cells11060951. doi: 10.3390/cells11060951.
PubMed
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| Genome-wide screening identifies Polycomb repressive complex 1.3 as an essential regulator of human naive pluripotent cell reprogramming. Collier AJ, Bendall A, Fabian C, Malcolm AA, Tilgner K, Semprich CI, Wojdyla K, Nisi PS, Kishore K, Roamio Franklin VN, Mirshekar-Syahkal B, D'Santos C, Plath K, Yusa K, Rugg-Gunn PJ. Sci Adv. 2022 Mar 25;8(12):eabk0013. doi: 10.1126/sciadv.abk0013. Epub 2022 Mar 25. PubMed |
| Extended intergenic DNA contributes to neuron-specific expression of neighboring genes in the mammalian nervous system. Jaura R, Yeh SY, Montanera KN, Ialongo A, Anwar Z, Lu Y, Puwakdandawa K, Rhee HS. Nat Commun. 2022 May 18;13(1):2733. doi: 10.1038/s41467-022-30192-z. PubMed |
| Inhibition of RNA Binding in SND1 Increases the Levels of miR-1-3p and Sensitizes Cancer Cells to Navitoclax. Lehmusvaara S, Haikarainen T, Saarikettu J, Martinez Nieto G, Silvennoinen O. Cancers (Basel). 2022 Jun 24;14(13). pii: cancers14133100. doi: 10.3390/cancers14133100. PubMed |
| Grafted hPSC-derived GABA-ergic interneurons regulate seizures and specific cognitive function in temporal lobe epilepsy. Upadhya D, Attaluri S, Liu Y, Hattiangady B, Castro OW, Shuai B, Dong Y, Zhang SC, Shetty AK. NPJ Regen Med. 2022 Aug 1;7(1):38. doi: 10.1038/s41536-022-00234-7. PubMed |
| Reduced calcium levels and accumulation of abnormal insulin granules in stem cell models of HNF1A deficiency. Gonzalez BJ, Zhao H, Niu J, Williams DJ, Lee J, Goulbourne CN, Xing Y, Wang Y, Oberholzer J, Blumenkrantz MH, Chen X, LeDuc CA, Chung WK, Colecraft HM, Gromada J, Shen Y, Goland RS, Leibel RL, Egli D. Commun Biol. 2022 Aug 2;5(1):779. doi: 10.1038/s42003-022-03696-z. PubMed |
| Systematic Functional Analysis of PINK1 and PRKN Coding Variants. Broadway BJ, Boneski PK, Bredenberg JM, Kolicheski A, Hou X, Soto-Beasley AI, Ross OA, Springer W, Fiesel FC. Cells. 2022 Aug 5;11(15):2426. doi: 10.3390/cells11152426. PubMed |
| Enhancer-promoter interactions can bypass CTCF-mediated boundaries and contribute to phenotypic robustness. Chakraborty S, Kopitchinski N, Zuo Z, Eraso A, Awasthi P, Chari R, Mitra A, Tobias IC, Moorthy SD, Dale RK, Mitchell JA, Petros TJ, Rocha PP. Nat Genet. 2023 Feb;55(2):280-290. doi: 10.1038/s41588-022-01295-6. Epub 2023 Jan 30. PubMed |
| ZnT8 Loss of Function Mutation Increases Resistance of Human Embryonic Stem Cell-Derived Beta Cells to Apoptosis in Low Zinc Condition. Sui L, Du Q, Romer A, Su Q, Chabosseau PL, Xin Y, Kim J, Kleiner S, Rutter GA, Egli D. Cells. 2023 Mar 15;12(6):903. doi: 10.3390/cells12060903. PubMed |
| Complex regulation of Eomes levels mediated through distinct functional features of the Meteor long non-coding RNA locus. Gil N, Perry RB, Mukamel Z, Tuck A, Buhler M, Ulitsky I. Cell Rep. 2023 May 30;42(6):112569. doi: 10.1016/j.celrep.2023.112569. PubMed |
| Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids. Huang B, Zeng Z, Li H, Li Z, Chen X, Guo J, Zhang CC, Schreiber ME, Vonk AC, Xiang T, Patel T, Li Y, Parvez RK, Der B, Chen JH, Liu Z, Thornton ME, Grubbs BH, Diao Y, Dou Y, Gnedeva K, Lindstrom NO, Ying Q, Pastor-Soler NM, Fei T, Hallows KR, McMahon AP, Li Z. bioRxiv. 2023 May 25:2023.05.25.542343. doi: 10.1101/2023.05.25.542343. Preprint. PubMed |
| The Junctophilin-2 Mutation p.(Thr161Lys) Is Associated with Hypertrophic Cardiomyopathy Using Patient-Specific iPS Cardiomyocytes and Demonstrates Prolonged Action Potential and Increased Arrhythmogenicity. Valtonen J, Prajapati C, Cherian RM, Vanninen S, Ojala M, Leivo K, Helio T, Koskenvuo J, Aalto-Setala K. Biomedicines. 2023 May 27;11(6):1558. doi: 10.3390/biomedicines11061558. PubMed |
| Optogenetic control of Wnt signaling models cell-intrinsic embryogenic patterning using 2D human pluripotent stem cell culture. Repina NA, Johnson HJ, Bao X, Zimmermann JA, Joy DA, Bi SZ, Kane RS, Schaffer DV. Development. 2023 Jul 15;150(14):dev201386. doi: 10.1242/dev.201386. Epub 2023 Jul 26. PubMed |
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