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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 44655 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-5X-3
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Backbone manufacturerGE Healtcare
- Backbone size w/o insert (bp) 4974
- Total vector size (bp) 7862
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namendoS
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Alt nameEndoglycosidase S
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SpeciesStreptococcus pyogenes
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Insert Size (bp)2878
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MutationNone
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GenBank IDAF296340
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Entrez GenendoS (a.k.a. SpyM3_1568)
- Promoter tac
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Tag
/ Fusion Protein
- Gst (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer 5' pGEX sequencing primer
- 3′ sequencing primer 3' pGEX sequencing primer (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEXndoS was a gift from Mattias Collin (Addgene plasmid # 44655 ; http://n2t.net/addgene:44655 ; RRID:Addgene_44655) -
For your References section:
Effect of SpeB and EndoS from Streptococcus pyogenes on human immunoglobulins. Collin M, Olsen A. Infect Immun. 2001 Nov . 69(11):7187-9. 10.1128/IAI.69.11.7187-7189.2001 PubMed 11598100
Map uploaded by the depositor.
