pLV-mitoGFP
(Plasmid #44385)

• Depositing Lab: Pantelis Tsoulfas
• Publication: Kitay et al Hum Mol Genet. 2013 Jan 24.

Backbone

• Vector backbone: pLV
• Backbone size w/o insert (bp): 6727
• Total vector size (bp): 7500
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Grow and plate in LB with low salt
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mitoGFP
• Alt name: Cox8 targeting sequence and GFP
• Species: H. sapiens (human)
• Insert Size (bp): 820
• GenBank ID: AK311934
• Entrez Gene: COX8A (a.k.a. COX, COX8, COX8-2, COX8L, MC4DN15, VIII, VIII-L)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Cox8 targeting sequence (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry