pUC-UAS1B8-TEF(504)
(Plasmid
#44372)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44372 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2623
- Total vector size (bp) 4056
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Modifications to backboneNone
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUAS1B8-TEF(504) promoter
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SpeciesYarrowia lipolytica
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Insert Size (bp)1433
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MutationN/A
- Promoter UAS1B8-TEF(504)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BstBI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer m13_forward20_primer
- 3′ sequencing primer m13_reverse_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Discrepancies between the Addgene QC sequence and the depositor's sequence should not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC-UAS1B8-TEF(504) was a gift from Hal Alper (Addgene plasmid # 44372 ; http://n2t.net/addgene:44372 ; RRID:Addgene_44372) -
For your References section:
Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Blazeck J, Liu L, Redden H, Alper H. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16. 10.1128/AEM.05763-11 PubMed 21926196
