Skip to main content
Addgene

pCsy4
(Plasmid #44252)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC19
  • Total vector size (bp) 3392
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    csy4 gene
  • Species
    P. aeruginosa
  • Promoter pLtetO-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer gagaaaagaattcaaaAGATCTaaagaggagaaaGGATCTATGGACCACTACCTC
  • 3′ sequencing primer agatccttactcgagttaTCAGAACCAGGGAACGAAACCTCCTTTGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCsy4 was a gift from Adam Arkin & Stanley Qi (Addgene plasmid # 44252 ; http://n2t.net/addgene:44252 ; RRID:Addgene_44252)

  • For your References section:

    RNA processing enables predictable programming of gene expression. Qi L, Haurwitz RE, Shao W, Doudna JA, Arkin AP. Nat Biotechnol. 2012 Oct;30(10):1002-6. doi: 10.1038/nbt.2355. Epub 2012 Sep 16. 10.1038/nbt.2355 PubMed 22983090
Related items:
Commonly requested with: