pdCas9-bacteria
(Plasmid #44249)

• Purpose: aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
• Depositing Lab: Stanley Qi
• Publication: Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.

Backbone

• Vector backbone: p15A vector
• Total vector size (bp): 6705
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: dCas9 (bacteria)
• Alt name: Catalytically inactive Cas9
• Species: S. pyogenes
• Insert Size (bp): 4107
• Mutation: D10A H840A (catalytically inactive)
• Promoter: pLtetO-1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: ttcaaaaGATCTAAAGAGGAGAAAGGATCTATGGATAAGAAATACTCAATAG
• 3′ sequencing primer: tgcctggagatccttactcgaGTTAGTCACCTCCTAGCTGACTCAAATCAAT

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest- XhoI
• Articles Citing this Plasmid:
  • 76 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Qi Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/qi/