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Addgene

D998-EGFR-ET1
(Plasmid #44186)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44186 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    BD Biosciences Clontech
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 8600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D998-EGFR-ET1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3306
  • Mutation
    EGFR amino acids 1−998
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
  • Promoter CVM
  • Tag / Fusion Protein
    • 3C cleavge site, protein C peptide tag, his6, SBP tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TTGGCACCAAAATCAACGGGACT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid expresses amino acids 1−998 of mature human EGFR and does not include the 24aa signal peptide sequence in GenBank reference sequence NP_005219.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    D998-EGFR-ET1 was a gift from Timothy Springer (Addgene plasmid # 44186 ; http://n2t.net/addgene:44186 ; RRID:Addgene_44186)

  • For your References section:

    Functional and structural stability of the epidermal growth factor receptor in detergent micelles and phospholipid nanodiscs. Mi LZ, Grey MJ, Nishida N, Walz T, Lu C, Springer TA. Biochemistry. 2008 Sep 30;47(39):10314-23. doi: 10.1021/bi801006s. Epub 2008 Sep 5. 10.1021/bi801006s PubMed 18771282
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