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Addgene

pCambia1300-PacI
(Plasmid #44183)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44183 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCambia1300
  • Backbone manufacturer
    http://www.cambia.org/daisy/cambia/2045/version/1/part/4/data/pCAMBIA1300.pdf?branch=main&language=default
  • Backbone size (bp) 8958
  • Modifications to backbone
    modified it to contain only one PacI site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note- the SpeI site located within the Kanamycin resistance cassette was destroyed (tested by digest). The fact that the plasmid is growing on Kanamycin indicates that the loss of the SpeI site does not affect the plasmid's function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCambia1300-PacI was a gift from Guiliang Tang (Addgene plasmid # 44183 ; http://n2t.net/addgene:44183 ; RRID:Addgene_44183)

  • For your References section:

    Construction of short tandem target mimic (STTM) to block the functions of plant and animal microRNAs. Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X. Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. Epub 2012 Oct 23. 10.1016/j.ymeth.2012.10.006 PubMed 23098881
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